FIG. 1.

LuOU are multicellular clusters derived from whole lung tissue (A, left). Polyglycolic acid and poly-l-lactic acid scaffolds (A, top) are loaded with LuOU (A, right), scale bar represents 1 mm. The loaded scaffolds are implanted subcutaneously into NSG hosts, where they remain 4 weeks before harvest as TELu (A, bottom), scale bar 5 mm. (B, C) IF staining for GFP (green) of SPC^GFP native lung (B) and TELu generated from SPC^GFP lung (C), scale bar 50 μm. (D, E) IF staining for GFP (green) and SPC (red), expressed by AEC2s, on SPC^GFP native lung (D) and TELu (E) generated from SPC^GFP lung, scale bar 100 μm. (F, G) IF for CC10 (red) to mark proximal airway club cells and GFP (green) in Actin^GFP native lung (F) and TELu (G) generated from Actin^GFP donor tissue (Actin^GFP TELu), scale bar 50 μm. (H, I) IF for CC10 (green) and SPC (red) for Actin^GFP native lung (H) and Actin^GFP TELu (I), scale bar 50 μm. (J, K) IF for T1α (AEC1s) (green) and SPC (red) in native lung (J) and TELu (K), scale bar 50 μm. (L, M) IF for GFP (green) and SPC (red) in Actin^GFP native lung (L) and Actin^GFP TELu, scale bar 100 μm. IF, immunofluorescent; LuOU, lung organoid unit; TELu, tissue-engineered lung. Color images available online at www.liebertpub.com/tec