FIG. 2.

(A, B) Alcian blue staining for goblet cells (blue) in native airway (A) and TELu (B), scale bar 50 μm. (C, D) IF for Sox2 (red), a marker for proximal airway progenitors, in native lung (C) and TELu (D), scale bar 100 μm. (E, F) IF for CGRP (red), pulmonary neuroendocrine cells in native lung (E) and TELu (F), scale bar 50 μm. (G, H) IF for p63 (red), basal cell marker, (G) native airway and (H) TELu, scale bar 100 μm. (I) Quantification of p63⁺ cells in native lung and TELu. (J, K) IF for β-tubulin-IV (red), ciliated cells, and CC10 (green) in (J) native lung and (K) TELu, scale bar 50 μm. (L) Quantification of percentage of CC10⁺ epithelial cells/total in native lung and TELu. (M, N) IF with β-tubulin-IV (red) to observe cilia morphology in native lung (M) and TELu (N), scale bar 50 μm. *p = 0.0074. Color images available online at www.liebertpub.com/tec