(A, B) IF for T1α (red) and SPC (green) in native lung (A) and TELu (B) generated from distal LuOU. (C, D) Alcian blue stain of native trachea (C) and TEtrach generated from proximal airway/tracheal organoid units (proximal LuOU) (D). (E, F) IF for β-tubulin-IV (red) in native trachea (E) and TEtrach (F). (G, H) IF for CC10 (green) in native trachea (G) and TEtrach (H). (I, J) IF for p63 (red) in native trachea (I) and TEtrach (J). (K, L) IF for E-Cadherin (green) in native trachea (K) and TEtrach (L). Scale bars 50 μm. TEtrach, tissue-engineered trachea. Color images available online at www.liebertpub.com/tec