FIG. 3.
In vitro oxygen calibration and validation using 19F-MRS relaxometry and FO oxygen techniques. (A) Calibration of PFCE-loaded TEGs demonstrated that R1 changes linearly with pO2. The slope and intercept of the calibration line changed with temperature. These values were used to generate the multiparametric calibration coefficients. The error bars are contained within the markers. (B) Plotting 19F-MRS against FO in vitro oxygen measurements showed a highly correlated relationship. These results validate the success of an accurate and reliable calibration (Pearson's R = 0.98, slope = 0.97, intercept = 4.3 mmHg). pO2, oxygen partial pressure; TEG, tissue-engineered graft.