Figure 2. Pterostilbene induces cell cycle arrest in DLBCL cell lines.

(A) Four DLBCL cell lines were treated for 24 h with pterostilbene (20, 40 and 60 μM) followed by propidium iodide staining and flow cytometric analysis. Values represent the mean ± SEM of three independent experiments performed in triplicate. (B) After treatment as in A, proteins were extracted from cultured SUDHL-4 and NU-DUL-1 cells and probed with appropriate dilutions of specific antibodies. Upper panels: Representative results of phospho-Histone H2A.X, chK2, Cyclin A2, CDK2, and cdc25A protein levels were as determined by a Western blot analysis. Lower panel: Quantitative results of related protein levels, which were adjusted with the actin protein level and expressed as multiples of induction beyond its own control. *p < 0.05, compared to the vehicle control group.