Table 2. List of primers used in the study.

Primer	Primer sequence (5′-3′)	Restriction site	Remarks
Pro564	AGCAGGACGAGAAAGCAATGAATGT	/	Forward primer to check pEM40 integration in Lactobacillus genome
Pro565	GCCGGTGTGGCGGAATTGGCAG	/	Reverse primer to check pEM40 integration
Pro7129	ATGTTCATGTAATCACTCCTTCTTAATTAC	/	Reverse primer to check insertion in multiple cloning site pLAB1301
Pro7130	ATAGGCTCCAAAAGGAGCCTTTAATTGTA	/	Forward primer to check insertion in multiple cloning site of pLAB1301
Pro7466	ATCCCGGGCCAAAATCATCCGTG	XmaI	Forward primer HR1 llp1 gene LGR-1
Pro7467	ATCCCGGGCATGATCGTCACTCCT	XmaI	Reverse primer HR1 llp1 gene LGR-1. Used for Southern hybridisation
Pro7468	ATGCGGCCGCGTGAGTCGAGTAAGCA	NotI	Forward primer HR2 llp1 gene LGR-1
Pro7469	ATGTCGACTGCTAGCGGTATATTCA	SalI	Reverse primer HR2 llp1 gene LGR-1
Pro8018	TTACACTCCGACTTCTAACCGC	/	Forward primer to check llp1 replacement with TcR cassette. Used for Southern hybridisation
Pro8019	CTAATCAGCGATGCTTAGTCG	/	Reverse primer to check llp1 replacement with TcR cassette
Pro8675	ATCCCGGGATGAAGAAGTGCGGCTA	XmaI	Forward primer complementation llp1gene
Pro8676	ATGAGCTCTCACTGAGGAGCGTT	SacI	Reverse primer complementation llp1 gene
Pro5880	CACCGTCGACCGAAGAAGAAATATTCA	SalI	Forward primer for full length llp1 gene for pET28 a(+)
Pro5881	ACTGGCGGCCGCTTAAGGCATAGGAGTAG	NotI	Reverse primer for full length llp1 gene for pET28 a(+)
S&P-00517	ATGTCGACAAGGGTGGCCGTCATCGTCAGG	SalI	Forward primer upstream of lectin-like domain of llp1 gene
S&P-00518	ATGCGGCCGCTTAATCTTCTACCTTCAAATGCGTG	NotI	Reverse primer upstream of lectin-like domain of llp1 gene
S&P-0044	TGGCAGCAGCCAACTCAGCTT	/	Reverse primer for MCS of pET28 a(+)
S&P-0045	TATAGGCGCCAGCAACCGCA	/	Forward primer for MCS of pET28 a(+)