FIG. 2.

Calibration images produced in Microsoft paint to portray the possible scenarios of protein localization in cells grown on different scaffolds. X and Y axes refer to the number of pixels in frame and Z-stack number, respectively. (Case 1) protein is localized to apical end with respect to cell fluorescence, (Case 2) protein is laterally expressed throughout the cell with respect to DAPI fluorescence, and (Case 3) protein that is expressed throughout the samples, even passed the lower limit of the fiber mat. The absolute bottom of the fiber mat is represented in the highlighted portion of the scaffold image (white), nanofiber mat (red), cell nucleus (blue), and tight junction protein, ZO-1 (cyan). DAPI, 4′,6-diamidino-2-phenylindole; ZO-1, zonula occludens-1. Color images available online at www.liebertpub.com/tec