FIG. 4.

Schematic showing the (A) Maximum projection, X and Y axes are number of pixels per line and lines per frame, respectively. (B) Y-Z cross-sections and (C) X-Z cross-sections that users can see for each channel while processing. The base of the scaffold is detected and highlighted (dotted lines at the bottom with red arrows). X and Z axes refer to the number of pixels in frame and Z-stack number, respectively. This is the same for the Y-Z axes as well. Color images available online at www.liebertpub.com/tec