FIG. 7.

Quantification of cell morphology and apicobasal polarization of cells grown on PLGA nanofiber scaffolds with or without conjugated laminin using Bio-LIME. Confocal maximum projection and X-Z slice of SIMS cells for 6 days on scaffolds. (A) PLGA scaffold, (B) PLGA + laminin scaffold. Scale bar, 10 μm. Nanofibers (red), DAPI (blue), ZO-1 tight junction protein (cyan), (C) SIMS cell morphology, (D) Localization of tight junction protein, ZO-1, of SIMS cells cultured on PLGA scaffolds, and PLGA + laminin scaffolds. ns, not significant; *p < 0.05, ***p < 0.001; unpaired t test. SIMS, submandibular immortalized mouse salivary gland epithelial. Color images available online at www.liebertpub.com/tec