FIG. 8.

Comparison of cell morphology and apicobasal polarization of cells grown on 250 nm versus sub-100 nm PLGA nanofiber scaffolds using Bio-LIME. Confocal maximum projection and X-Z slice of SIMS cells cultured for 6 days on scaffolds. (A) 250 nm PLGA scaffold, (B) sub-100 nm PLGA scaffold. Scale bar, 10 μm. Nanofibers (red), DAPI (blue), ZO-1 tight junction protein (cyan). (C) SIMS cells morphology, (D) Protein localization analysis of cells cultured on 250 nm diameter PLGA scaffolds and sub-100 nm diameter PLGA scaffolds. ns, not significant, *p < 0.05; unpaired t test. Color images available online at www.liebertpub.com/tec