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Tau oligomers detected with T22 (green) and total tau levels labeled with Tau-5 (red) in the cerebellum of control Htau (A–C) and TBI oligomer-injected Htau mice (D–F). Total fluorescence of individual cells labeled with T22, corrected for cell size and background fluorescence, showed that TBI tau oligomer-injected Htau brain samples had significantly higher levels of tau oligomers in the cerebellum (p = 0.0005) (G). Htau, human tau; Olig, oligomer; TBI, traumatic brain injury.
