TABLE 3.
Carbon metabolism genes differentially expressed between the isogenic fabT deletion mutant and the wild-type strain MGAS2221 grown at 35°C
| Locus tag | Gene | Fold changea | Growth phaseb | Function |
|---|---|---|---|---|
| Pyruvate hub enzymes | ||||
| M5005_Spy0751 | acoA | 2.5 | ME | Pyruvate dehydrogenase E1 component, alpha subunit |
| M5005_Spy0752 | acoB | 2.5 | ME | Pyruvate dehydrogenase E1 component, beta subunit |
| M5005_Spy0753 | acoC | 2.4 | ME | Pyruvate dehydrogenase, dihydrolipoamide acyltransferase (E2) component |
| M5005_Spy0755 | acoL | 2.4 | ME | Pyruvate dehydrogenase, dihydrolipoamide dehydrogenase (E3) component |
| M5005_Spy1569 | pfl | 1.8 | ME | Pyruvate formate lyase |
| M5005_Spy1841 | sdhB | 6.3 | ME | Serine dehydratase beta chain |
| M5005_Spy1842 | sdhA | 6.0 | ME | Serine dehydratase alpha chain |
| Genes involved in fermentation pathways | ||||
| M5005_Spy0873 | ldh | 2.3 | SP | l-Lactate dehydrogenase |
| M5005_Spy0039 | adh2 | −4.3 | ME | Acetaldehyde dehydrogenase |
| M5005_Spy0040 | adhA | −3.5 | ME | Alcohol dehydrogenase |
| M5005_Spy0039 | adh2 | −3.5 | SP | Acetaldehyde dehydrogenase |
| M5005_Spy0040 | adhA | −4.9 | SP | Alcohol dehydrogenase |
| M5005_Spy0094 | ackA | −1.6 | SP | Acetate kinase |
| M5005_Spy0851 | pta | −1.6 | SP | Phosphotransacetylase |
| Upregulated genes involved in entry of carbon sources into the Embden-Meyerhof-Parnas (glycolysis) pathway | ||||
| M5005_Spy0661 | fruB | 3.3 | SP | l-Phosphofructokinase |
| M5005_Spy0938 | pgmA | 1.6 | SP | Phosphoglucomutase |
| M5005_Spy1538 | pmi | 1.6 | ME | Mannose-6-phosphate isomerase |
| Downregulated genes involved in entry of carbon sources into the Embden-Meyerhof-Parnas (glycolysis) pathway | ||||
| M5005_Spy0903 | oadB | −2.5 | SP | Oxaloacetate decarboxylase beta subunit |
| M5005_Spy0905 | citD | −1.9 | SP | Citrate lyase subunit gamma |
| M5005_Spy0906 | citE | −1.9 | SP | Citrate lyase subunit beta/citryl-CoA lyase subunit |
| M5005_Spy0907 | citF | −2.0 | SP | Citrate lyase subunit alpha/citrate CoA-transferase |
| M5005_Spy0908 | citX | −2.4 | SP | Citrate lyase activation subunit |
| M5005_Spy1062 | malA | −2.1 | SP | Maltodextrose utilization protein |
| M5005_Spy1063 | malD | −2.1 | SP | ABC transporter transmembrane subunit |
| M5005_Spy1064 | malC | −2.0 | SP | Maltose/maltodextrin transporter membrane protein |
| M5005_Spy1067 | malX | −2.0 | SP | Maltose/maltodextrin transporter subunit |
| M5005_Spy0832 | maeP | −11.3 | SP | Putative malate transporter |
| M5005_Spy0833 | maeE | −10.0 | SP | Malic enzyme |
| M5005_Spy1379 | glpF | −4.4 | SP | Glycerol uptake facilitator |
| M5005_Spy1380 | glpO | −4.6 | SP | Glycerol-3-phosphate dehydrogenase |
| M5005_Spy1381 | glpK | −5.5 | SP | Glycerol kinase |
| M5005_Spy1542 | scrA | −2.4 | SP | Sucrose-specific transporter PTS system subunit II |
| M5005_Spy1543 | scrB | −2.4 | SP | Glycosyl hydrolase involved in sucrose utilization |
| M5005_Spy1544 | scrR | −2.4 | SP | Putative transcriptional regulator involved in sucrose utilization |
a
Fold change in the fabT mutant relative to the wild type.
b
ME, mid-exponential growth phase; SP, stationary growth phase.