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. 2016 Nov 18;84(12):3290–3301. doi: 10.1128/IAI.00630-16

FIG 6.

FIG 6

(A) Depurination of rat liver ribosomes by purified 10×His-tagged WT Stx1A1 and Stx2A1 and 10×His-tagged R170A variants. (B) Depurination of rat liver ribosomes by purified 10×His-tagged WT Stx1A1 and Stx2A1 and 10×His-tagged R176A and R172A/R176A variants. Rat liver ribosomes (7 pmol) were incubated with different concentrations of WT Stx1A1 or Stx2A1 or R170A, R176A, or R172A/R176A variants at 30°C for 10 min. Total rRNA (375 ng) was used to quantify the relative levels of depurination by qRT-PCR. The y axis shows the fold change in depurination of toxin-treated samples over the control samples without toxin treatment (NT). The error bars represent SE (n = 3 replicates). The means of WT Stx1A1 and Stx2A1 and their variants were significantly different using a two-sample t test (**, P < 0.01, ****, P < 0.0001 [means compared to the respective WT]; ##, P < 0.01, ####, P < 0.0001 [means compared between Stx1A1 and Stx2A1]; NS, not significant).