(A) Interaction of rat liver ribosomes with 10×His-tagged WT Stx1A1 and 10×His-tagged R176A and R172A/R176A variants. (B) Interaction of rat liver ribosomes with 10×His-tagged WT Stx2A1 and 10×His-tagged R176A and R172A/R176A variants. WT Stx1A1, WT Stx2A1, and R176A and R172A/R176A variants were captured on an NTA chip at 1,800 RU. Different concentrations of rat liver ribosomes were passed over the surface as analytes. (C) Interaction of rat liver ribosomes with 10×His-tagged WT Stx1A1 and 10×His-tagged R170A. (D) Interaction of rat liver ribosomes with 10×His-tagged WT Stx2A1 and 10×His-tagged R170A. 10×His-Stx1A1, 10×His-Stx2A1, and 10×His-R170A variants were captured on an NTA chip at 1,800 RU. Different concentrations of rat liver ribosomes were passed over the surface as analytes, as shown.