TABLE 2.
Stalk interaction parametersa
| Toxin | kon (M−1 s−1) | koff (s−1) | KD (M) |
|---|---|---|---|
| Stx1A1 | |||
| WT | 1.53 × 106 ± 0.76 × 106A | 2.29 × 10−3 ± 0.56 × 10−3C | 1.50 × 10−9 ± 0.59 × 10−9E |
| R170A | 1.08 × 106 ± 0.55 × 106B | 2.04 × 10−3 ± 0.23 × 10−3D | 1.89 × 10−9 ± 0.52 × 10−9F |
| Stx2A1 | |||
| WT | 1.28 × 106 ± 0.23 × 106G | 2.34 × 10−3 ± 0.45 × 10−3I | 1.84 × 10−9 ± 0.21 × 10−9K |
| R170A | 1.39 × 106 ± 0.08 × 106H | 2.55 × 10−3 ± 0.28 × 10−3J | 1.83 × 10−9 ± 0.31 × 10−9L |
a
The letters indicate statistical comparisons, where means were not significantly different between A and B, between C and D, between E and F, between G and H, between I and J, and between K and L as determined using a two-sample t test.