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. 2016 Nov 18;84(12):3350–3357. doi: 10.1128/IAI.00708-16

FIG 6.

FIG 6

IL-10 and TNF-α expression levels in peritoneal macrophages of hamsters and mice. Peritoneal macrophages of hamsters were challenged with leptospires or with leptospires plus Pam3CSK4 for 24 h. Peritoneal macrophages from WT, TLR2−/−, and TLR4−/− mice were challenged with leptospires for 24 h, and the mRNA levels of IL-10 and TNF-α were quantified by RT-qPCR. (A and B) IL-10 (A) and TNF-α (B) mRNA levels in peritoneal macrophages of WT, TLR2−/−, and TLR4−/− mice. (C) IL-10 and TNF-α mRNA levels in peritoneal macrophages of hamsters. The results were normalized to the expression level of the housekeeping gene GAPDH. Bars show mean levels of cytokines ± standard deviations, and differences were analyzed by one-way ANOVA. *, P < 0.05; ns, none significant.