TABLE 2.
Relative normalized expression levels of cytokinesa
| Organ | Presence of Pam3CSK4 | Mean expression level (fold induction) ± SE (n = 3) |
|
|---|---|---|---|
| IL-10 | TNF-α | ||
| Kidney | − | 0.0343 ± 0.0272 | 0.1668 ± 0.1916 |
| + | 0.2349 ± 0.2104* | 0.1719 ± 0.0626 | |
| Liver | − | 1.6743 ± 0.4519 | 8.5212 ± 1.9813 |
| + | 12.8796 ± 7.0677* | 8.5382 ± 1.7210 | |
| Lung | − | 1.3286 ± 0.5489 | 2.9089 ± 0.8059 |
| + | 14.2053 ± 10.9635* | 16.1554 ± 6.2201* | |
a
Hamsters were intraperitoneally infected with leptospires either alone or mixed with Pam3CSK4. The expression levels of IL-10 and TNF-α in each tissue were quantified by RT-qPCR at 24 h p.i. *, P < 0.05 for the group coinjected with leptospires and Pam3CSK4 versus infected controls as determined by one-way ANOVA followed by the Newman-Keuls test.