TABLE 3.
Relative normalized expression levels of cytokines in micea
| Organ | Mouse type | Mean expression level (fold induction) ± SE (n = 3) |
|
|---|---|---|---|
| IL-10 | TNF-α | ||
| Kidney | TLR2−/− | 4.3941 ± 1.8864 | 53.4172 ± 18.3277* |
| WT | 6.5701 ± 3.2300 | 19.8598 ± 11.9571 | |
| Liver | TLR2−/− | 3.9898 ± 1.6002 | 6.7567 ± 5.3288 |
| WT | 6.8564 ± 3.7698 | 1.1072 ± 0.1316 | |
| Lung | TLR2−/− | 1.4039 ± 0.4742* | 3.1589 ± 1.3706* |
| WT | 3.1605 ± 0.8553 | 0.8719 ± 0.2210 | |
a
Wild-type and TLR2-deficient mice were intraperitoneally infected with leptospires. The expression levels of IL-10 and TNF-α in each tissue were quantified by RT-qPCR at 4 days p.i. *, P < 0.05 for TLR2−/− mice versus WT mice by one-way ANOVA followed by the Newman-Keuls test.