FIG 2.

Motility of C. jejuni wild-type 81-176, Δpgp1, Δpgp2, Δ1228, and complemented strains. (A) As assayed by measuring halo diameters in soft agar plates, the Δpgp1, Δpgp2, and Δ1228 mutants exhibited 81.0%, 66.3%, and 85.1% of wild-type motility, respectively. Complementation of the Δ1228 mutant (Δ1228c) showed restoration of wild-type motility. Standard error of the mean was calculated from 9 measurements. The asterisks indicate a statistically significant difference using the unpaired Student t test, **** indicating a P value of <0.0001. (B) Motility of the wild-type, mutant, and complemented C. jejuni strains in liquid MH medium recorded over 6 s with a time-lapse of 0.2 s/frame. The velocity (micrometers per second) was calculated by measuring the distance moved by individual cells between frames, divided by the number of frames over which the movement was tracked. Statistical significance was determined using a Kruskal-Wallis test (P < 0.05).