FIG 3.

Intramuscular vaccination with NEAT domains and alum. (A) Survival curves for mice vaccinated with adjuvant alone (alum) or alum plus a NEAT cocktail (4 μg of each NEAT domain, for 20 μg total) after challenge with 5× LD₅₀ of B. anthracis Sterne strain spores. For alum versus the NEAT cocktail, the P value was <0.0001 as determined by a log rank (Mantel-Cox) test, and the P value was <0.0001 as determined by a Wilcoxon test. (B) Comparison of the total B. anthracis CFU recovered from mouse organs (lungs, spleen, kidneys, heart, and gastrointestinal [GI] tract) treated with alum (black bars) alone versus alum with a NEAT cocktail (gray bars) after necropsy of animals on day 70. Asterisks indicate statistical significance (P < 0.01) as determined by one-way analysis of variance.