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Analysis of heme-binding properties of HalN mutants. Increasing concentrations of heme (inset) were incubated with wild-type (WT) HalN (A), HalNY112A (B), HalNF116A (C), HalNY112A,F116A (D), and heme alone (E) for 30 min, and spectral scans of the reaction mixtures were collected to assess the intensity of the Soret band, the spectral signal associated with heme binding.
