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Analysis of the heme acquisition properties of HalN mutants. Wild-type HalN (A), HalNY112A (B), HalNF116A (C), and HalNY112A,F116A (D) were incubated with hemoglobin (black lines) or buffer alone (gray lines) for 30 min at room temperature and assessed for their ability to actively scavenge heme from hemoglobin by the intensity of the Soret band.
