TABLE 1.

Bacterial strains used in this study

Strain	Plasmid	Property	Reference(s)
E. coli BL21	pgst-halN	Encodes the NEAT domain (amino acids 29–152) of HalN	53
E. coli BL21	pgst-isdC	Encodes the NEAT domain (amino acids 32–212) of IsdC	57
E. coli BL21	pgst-isdX1N	Encodes the NEAT domain (amino acids 27–152) of IsdX1	49
E. coli BL21	pgst-isdX2FL	Encodes the NEAT domains of IsdX2 (amino acids 30–859)	49
E. coli BL21	pgst-bslKN	Encodes the NEAT domain (amino acids 46–165) of BslK	52
E. coli BL21	pgst-halNY112A	Encodes HalN with an alanine substitution for tyrosine 112	This study
E. coli BL21	pgst-halNF116A	Encodes HalN with an alanine substitution for phenylalanine 116	This study
E. coli BL21	pgst-halNY112A,F116A	Encodes HalN with alanine substitutions for tyrosine 112 and phenylalanine 116	This study
B. anthracis Sterne 34F2	pUTE657	Avirulent, attenuated, nonencapsulated strain (Specr)	92, 93