FIG 3.

TLR2 activation is involved in F. alocis-induced phosphorylation of both ERK1/2 and p38 MAPK. Neutrophils were unchallenged (basal), challenged with F. alocis (MOI of 10, 15 min), or pretreated with either anti-TLR2 MAb or isotype control (isotype-Ctrol), followed by F. alocis challenge. Cells were lysed and proteins separated by SDS-PAGE and immunoblotted for phospho-p38 (P-p38) or phospho-ERK1/2 (P-ERK1/2). Blots were stripped and reblotted for total p38 (p38) or total ERK1/2 (ERK1/2), respectively. (A) Representative immunoblot of 4 independent experiments. (B) Densitometric analysis of the 4 immunoblots for P-ERK1/2/total ERK1/2. (C) Densitometric analysis of the 4 immunoblots for P-p38 MAPK/total p38 MAPK. Data are expressed as mean fold changes ± SEM over the basal level of the phosphorylated/total kinase ratio.