FIG 2.

The ΔCD1492 mutant has a hypersporulation phenotype. (A) Representative phase-contrast micrographs of the parent strain (630Δerm) and the ΔCD1492 mutant (MC674) grown on 70:30 sporulation agar for 24 h. Open arrowheads indicate phase-bright spores. (B) Ethanol-resistant spore formation frequency per total viable cells of the 630Δerm and ΔCD1492 mutant strains collected from 70:30 sporulation agar after 24 h. Sporulation frequencies were calculated as described for each assay in Materials and Methods. The mean values and the standard errors of the means are shown. *, P ≤ 0.05 by a two-tailed Student t test.