FIG 3.

Expression of key early sporulation regulators and putative sporulation kinases in the ΔCD1492 mutant. Transcriptional analysis of spo0A, sigF, and sigE (A) and predicted sporulation kinase CD1579 and CD2492 expression (B) in the ΔCD1492 mutant (MC674) relative to that in parent strain 630Δerm. Cultures were grown on 70:30 sporulation agar for 12 h, RNA was harvested, cDNA was prepared, and qRT-PCR was performed with gene-specific primers as outlined in Materials and Methods. The mean values and the standard errors of the means of at least three biological replicates are shown. *, P ≤ 0.05 by two-tailed Student t test.