TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or features | Source, construction, and/or reference |
|---|---|---|
| E. coli strains | ||
| HB101 | F− mcrB mrr hsdS20 (rB− mB−) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20 | B. Dupuy |
| MC277 | HB101 containing pRK24 and pMC211 | 28 |
| MC527 | HB101 containing pRK24 and pMC381 | This study |
| MC559 | HB101 containing pRK24 and pMC386 | This study |
| MC779 | HB101 containing pRK24 and pMC539 | This study |
| C. difficile strains | ||
| 630 | Clinical isolate | 65 |
| 630Δerm | Erms derivative of strain 630 | N. Minton, 66 |
| MC282 | 630Δerm pMC211 | 28 |
| MC587 | 630Δerm pMC386 | This study |
| MC674 | 630Δerm ΔCD1492 | This study |
| MC729 | 630Δerm ΔCD1492 pMC211 | This study |
| MC730 | 630Δerm ΔCD1492 pMC386 | This study |
| MC771 | 630Δerm ΔCD1492 pMC539 | This study |
| Plasmids | ||
| pRK24 | Tra+ Mob+ bla tet | 67 |
| pUC19 | Cloning vector, bla | 68 |
| pMTL-SC7315 | For allelic exchange in nonepidemic C. difficile strains | N. Minton, 26 |
| pMC123 | E. coli-C. difficile shuttle vector, bla catP | 69 |
| pMC211 | pMC123 PcprA | 28 |
| pMC381 | pMTL7315 ΔCD1492 cassette | This study |
| pMC386 | pMC211 with CD1492 | This study |
| pMC538 | pUC19 CD1492 H668A | This study |
| pMC539 | pMC211 with CD1492 H668A | This study |