TABLE 3.
The C. difficile CD1492 mutant forms more ethanol-resistant spores on 70:30 sporulation agar
| Strain | No. of CFU/ml |
Sporulation frequencyd | % Sporulation | ||
|---|---|---|---|---|---|
| Vegetative cellsa | Sporesb | Total cellsc | |||
| 630Δerm | 1.49 × 108 ± 2.13 × 107e | 3.60 × 107 ± 6.18 × 106 | 1.85 × 108± 1.61 × 107 | 2.23 × 10−1 ± 7.28 × 10−2 | 22.3 ± 7.28 |
| ΔCD1492 | 1.64 × 107 ± 3.88 × 106 | 5.33 × 107 ± 5.98 × 106 | 6.97 × 107 ± 8.03 × 106 | 7.76 × 10−1 ± 5.18 × 10−2 | 77.6 ± 5.18 |
a
The numbers of vegetative cells are the numbers of CFU recovered on BHIS medium plates.
b
The number of spores is the total number of CFU that survived ethanol treatment as described in Materials and Methods and were recovered on BHIS medium plates supplemented with 0.1% taurocholate.
c
The total cell numbers include both vegetative cells and spores.
d
The sporulation frequency was calculated as the number of ethanol-resistant spores divided by the total number of cells.
e
Values are means and standard errors of the means.