TABLE 4.
Comparison of gene expression and phenotypes of rstA and CD1492 null mutants
| Trait | Product | Fold changef vs parent |
|
|---|---|---|---|
| CD1492 | rstAa | ||
| Phenotypeb | |||
| Sporulation frequencyc | ↑ 2.4 | ↓ 20.1 | |
| Virulenced | ↓ 2.4 | ↑ 1.3 | |
| Gene expressionb | |||
| CD2492 | Sporulation sensor kinase | ↓ 1.2 | ↑ 2.4 |
| CD1579 | Sporulation sensor kinase | ↓ 1.9 | ↑ 4.9 |
| CD1492 | Sporulation sensor kinase | —e | ↑ 2.4 |
| rstA | Sporulation/sigD regulator | ↓ 3.6 | —e |
| spo0A | Sporulation master regulator | ↑ 1.9 | ↓ 1.4 |
| sigF | Sporulation sigma factor | ↑ 2.9 | ↓ 2.2 |
| sigE | Sporulation sigma factor | ↑ 2.3 | ↓ 6.4 |
| sigD | Motility sigma factor | ↓ 6.3 | ↑ 2.5 |
| fliC | Flagellar component | ↓ 3.1 | ↑ 3.5 |
| tcdR | Toxin sigma factor | 0.0 | ↑ 2.9 |
| tcdA | Toxin A | ↓ 2.0 | ↑ 4.0 |
| tcdB | Toxin B | ↓ 1.1 | ↑ 3.5 |
a
rstA values obtained under the same experimental conditions, previously reported (24).
b
All mean fold changes reported are relative to parent strain 630Δerm. Bold values are statistically significantly different (P ≤ 0.05 by two-tailed Student t test).
c
As determined by phase-contrast microscopy.
d
Defined here as fold change in time to morbidity in hamsters.
e
No significant transcript levels were detected in null mutants.
f
Ratios of the mean relative transcript levels for mutant and wild-type strains.