FIG 4.

H. diminuta antigens recruit monocytic-like cells. Peritoneal cells were obtained 24 h after HdAg (1 mg), LPS (1 μg), or PBS injection. Panel A demonstrates changes in cell distribution based on forward and side scatter (FSC and SSC, respectively) parameters. Distinct populations of CD11b⁺ Gr-1⁻ or Gr-1^hi and Gr-1^lo cells are apparent (B), with a significant increase in CD11b⁺ Gr-1^lo infiltrating cells in response to HdAg. Characterization of Ly6C and CCR2 (C) and F4/80 (D) defined this population as CD11b⁺ Gr-1^lo Ly6C⁺ F4/80⁺ monocyte-like cells. (E) H&E staining of the sorted cells revealed a polymorphonuclear morphology in the F4/80⁻ Gr-1^hi cells consistent with neutrophils, whereas the F4/80⁺ Gr-1^lo cells were of monocyte/macrophage appearance and were predominantly PD-L1⁺ PD-L2⁻ (n = 4 to 6).