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Primers used for site-directed mutagenesis
| Primer | Sequence (5′ to 3′)a |
|---|---|
| RalFRt E100A | CATTTAAATTACCGGGCGCCGCTCAAAAAATCGATAGG |
| Arf6T27N | CGCGGCCGGCAAGAACACAATCCTGTAC |
| Arf6N48I | CCACTGTGGGTTTCATCGTGGAGACGGTGAC |
| Arf6N122I | CCTCATCTTCGCCATCAAGCAGGACCTGC |
Underlined letters indicate a mutated codon.
