FIG 1.

Responses of WT and IL-17RA-knockout animals to pulmonary P. aeruginosa infection. (a) Pulmonary bacterial burdens in animals chronically infected with YH5 at 2 weeks postinfection. Lines indicate median values. P value was determined with the Mann-Whitney test from pooled results of two experiments, each with 10 mice per group. (b) Kaplan-Meir survival curves of WT (circles) or IL-17RA knockout (KO) animals (squares) infected with the NH57388A strain (n = 10 per group). Significant differences were determined by log rank test; P = 0.0092. (c) Weight changes in animals (n = 10) remaining chronically infected at 14 days following infection with the YH5 strain; results are for WT (circles) or IL-17RA KO animals (squares). Each point represents the mean weight at that time; error bars show SEM. Differences between the groups are significant at a P value of <0.0001 by repeated-measures ANOVA. (d, e) Hematoxylin and eosin (H&E)-stained lung sections from infected WT mice 2 weeks after infection with YH5 strain. Arrows show agar beads. Scale bars show 100 μm (d) or 50 μm (e). (f) Large (>50 cells deep) monocytic accumulations were observed in lungs from IL-17RA KO mice. Scale bar shows 10 μm. (g) Histological scores for indicated animals 2 weeks after infection with YH5 strain. P = 0.7266 by Mann-Whitney test.