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. 2016 Nov 18;84(12):3507–3516. doi: 10.1128/IAI.00717-16

FIG 3.

FIG 3

Lung parenchymal responses to P. aeruginosa infection. (a) Representative intracellular expression of IL-17A in lung parenchymal cells 2 weeks following instillation of sterile or P. aeruginosa-laden (strain NH57388A) beads. Numbers show percentages of total cells in ringed areas. (b) Percentages of total lung parenchymal cells expressing IL-17A 2 weeks after introduction of sterile or P. aeruginosa-laden beads. Each symbol represents an individual animal; lines show median values. P value was determined by Mann-Whitney test. (c) Representative expression of intracellular IL-17A in CD3+ and CD3 populations in lungs 2 weeks following P. aeruginosa infection as assessed by flow cytometry. (d, e) Data are as described in the legend to panel B but show the percentages of CD3+ (d) and CD3 (e) cells expressing intracellular IL-17A. (f to h) Flow cytometry results for CD3+ IL-17A+ cells expressing the indicated markers; boxed areas are deemed positive relative to results for isotype controls. Figures show percentages of the total CD3+ IL-17A+ population expressing the indicated markers. (i, j) Data are as described for panels F to H but with gating on CD3 IL-17A+ cells.