FIG 4.

Characteristics of B cells in lungs of animals instilled with P. aeruginosa-laden beads. (a to d) Two weeks after transtracheal instillation of P. aeruginosa-laden agar beads, the lungs were homogenized and cells polyclonally stimulated, extracellularly stained for CD3, B220, and CD43, and then permeabilized and stained for intracellular IL-17A, followed by flow cytometry. (a, b) Representative plots of the expression of B220 and IL-17A by live CD3⁻ cell populations. (b) Values above gates represent percentage of total IL-17A⁺ cells in each gate. (c, d) Representative plots of expression of IL-17A and CD43 by CD3⁻ B220⁺ cells. (d) Values in quadrants represent percentages of total cells. Results shown are representative of two separate experiments. (e, f) Lung sections from animals 2 weeks following infection with NH57388A were immunostained for B cells using B220 staining (e) or for T cells with Thy1.2 staining (f). Immunostaining is indicated in red, and boxed areas are shown enlarged to the right. Nuclei are counterstained green.