TABLE 2.
Primers used in the study
| Primer | Sequencea | Application | Source |
|---|---|---|---|
| dacA-F | TGCGGTTGGTATTTCAGAAG | dacA qRT-PCR | This study |
| dacA-R | TTTCTTTTGAAAGCGTGTGC | dacA qRT-PCR | This study |
| gdpP-F | TTAGTCGATGGGCAACTGAG | gdpP qRT-PCR | This study |
| gdpP-R | TTAATTGGGCACGATAACCA | gdpP qRT-PCR | This study |
| sigA-F | AACTGAATCCAAGTGATCTTAGTG | sigA qRT-PCR | 65 |
| sigA-R | TCATCACCTTGTTCAATACGTTTG | sigA qRT-PCR | 65 |
| CMG2 | CCCTGCAGATTGATAAATTGGGGATAAAGGAA | 500 bp 5′ dacA | This study |
| CMG4 | CCGTCGACTTATTTCACACCTTTCTTTTGAAAG | 3′ dacA | This study |
| CMG7 | CCCTGCAGGCCGATGCGAATATGAC | 500 bp 5′ gdpP | This study |
| CMG9 | CCGTCGACTCATGCATCTTCACTCCTAC | 3′ gdpP | This study |
| CMG10 | CCGAATTCGCGCGACTTTTCATTT | SAUSA300_2126 | This study |
| CMG11 | CCGTCGACTAAAATTTCCTTCTATTACTTTCTATTTCT | SAUSA300_2126 | This study |
| CMG12 | CCGAATTCAAATTAAATCACTTAATGTTAAACAAGGTGA | SAUSA300_2298 | This study |
| CMG13 | CCGTCGACTTATTCATGATTGATACTATTATCTGC | SAUSA300_2298 | This study |
| CMG14 | CCGAATTCATGTACACAAGGAGTGAGT | SAUSA300_2360 | This study |
| CMG15 | CCGTCGACTTATTGACGAGAATCAACTTC | SAUSA300_2360 | This study |
a
Primer oligonucleotide sequences are provided in the 5′-to-3′ orientation. Italics indicate nonhomologous sequences added for cloning.