FIG 6.

AFN-1252 arrested the division of intracellular L. monocytogenes. L. monocytogenes-infected HeLa cells were labeled with anti-Listeria-specific rabbit IgG antibodies, Alexa Fluor 488 (green)-conjugated goat anti-rabbit IgG, and Alexa Fluor 594 (red)-conjugated phalloidin. (Top) L. monocytogenes staining (green). (Middle) Cellular actin staining (red). (Bottom) Merges of the two images from the top and middle. (A) Untreated HeLa cells infected with L. monocytogenes for 20 h. (B) AFN-1252 (50 μM)-treated HeLa cells infected with L. monocytogenes for 20 h.