FIG 2.

Comparison of ex vivo PBMC proliferation among cell-mediated immunity profiles. PBMCs were stimulated by L. infantum antigen (Leish) for 5 days or with concanavalin A (ConA) or medium alone (Medium) for 72 h. Proliferation was then measured by BrdU colorimetric assay. CTL, uninfected dogs; PL, parasite load (number of parasites in the volume of lymph node aspirate which yielded 10 ng of DNA); OD, optical density. *, P < 0.05; ***, P < 0.001 (ANOVA parametric test for proliferation and nonparametric Mann-Whitney test for PL).