Fig. 2.
Developmental PCD is decreased in both Fmr1 mutant strains. TUNEL assays were performed on coronal sections. a Representative sections imaged at ×10 from somatosensory neocortex (NC), hippocampus (HC), caudate/putamen (CPu), and basolateral amygdala (BLA) are depicted. Green TUNEL-positive nuclei are visible and are indicated by white arrowheads. Nuclei counterstained with DAPI appear blue. CA1, CA3, dentate gyrus (DG) regions of HC, lateral ventricle (LV), dorsolateral (LaDL), and ventrolateral (LaVL) nucleus of the amygdala are labeled. Scale bar, 100 μm. b Quantification of total TUNEL-positive nuclei from NC, HC, CPu, and BLA in 3–4 nonserial coronal sections is demonstrated. Values are expressed as means + SD. * p < 0.05 vs. control; † p < 0.01 vs. control; n = 3–4 animals per cohort.