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. 2016 Oct 6;167(2):512–524.e14. doi: 10.1016/j.cell.2016.08.070

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© 2016 The Francis Crick Institute

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Figure S6 — Additional Analysis of the Rix1 Particles, Related to Figure 5

(A) Whole cell extracts were prepared from cells expressing Rix1-5FLAG. Rix1-5FLAG was immunoprecipitated with anti-FLAG antibody. Co-immunoprecipitated proteins were analyzed by SDS-PAGE and Coomassie staining. Indicated protein bands were identified by mass spectrometry.

(B) Co-immunoprecipitation of Rix1-5FLAG and Ytm1-3HA. Whole cell extract (input) were prepared from cells treated with Rbin-1 (1 μM) for indicated time. Rix1-5FLAG was immunoprecipitated with anti-FLAG antibodies (IP: FLAG). The indicated proteins were analyzed by SDS-PAGE and western blotted using anti-FLAG and anti-HA antibodies. Representative images are shown. The grouping of images from different parts of the same gel is indicated by dividing lines. The graphs show relative amount of Ytm1 in IP samples. (Error bars represent SD (n = 3)).