Figure 2.

Areas of scoring demyelination, luxol fast blue stain and immunohistochemistry in different brain regions of ICR and BALB/c mice with and without cuprizone (Cupri) exposure.

(A) Regions of the mouse brain where tissues were histologically analyzed. (B) Demyelination scores in different brain regions. Higher scores indicate more severe demyelination. (C, D) Representative images of demyelination determined by LFB stain (C) and immunolabelling with CNPase (D). Scoring of demyelination was done in CNPase or LFB-stained sections by three independent observers. (B–D) Feeding of Cupri for 6 weeks induced demyelination in both BALB/c and ICR mice, observed predominantly in the corpus callosum and cerebral cortex. Demyelination in the deep gray matter was observed only in the BALB/c mice, but not in ICR mice. Data in B are shown as the mean ± SE. *P < 0.05, **P < 0.01, ***P < 0.001 (one-way analysis of variance followed by Tukey's post-hoc test). mCC: Middle corpus callosum; lCC: lateral corpus callosum. Scale bars: 200 μm.