TABLE 4.

PHISTc genes

Gene identificationa	Other gene identification(s) or name(s)b	Presence of PEXELc	Gene statusd	Molecular mass (kDa)e	Length (aa)e	KOf	Description (reference[s] or source)g
PF3D7_0202100	PFB0105c, LSAP2	x		25.4	216		Not recognized by pooled immune sera (98); continuously upregulated, with predicted TM, granular pattern in immunofluorescence assays (IFA) of older parasites, expressed in liver stages (130); located at periphery in liver stage parasites similarly to circumsporozoite protein (CSP) (131); polymorphic gene with SNPs (80); differentially expressed in parasites under lumefantrine pressure (81)
PF3D7_0219700	PFB0900c, PF02_0184, GEXP20			35.3	295
PF3D7_0219800	PFB0905c, PF02_0185	x		31.9	263
PF3D7_0424000	PFD1140w, MAL4P1.223	x		35.2	296	x	Antigenic properties, antibody recognition in several reports, potential vaccine candidate (29, 49, 111, 132, 133); stronger expression in culture supplemented with albumin instead of human serum (119); String database mining suggests interaction with var2csa and PFB0115w (84); often referred to together with PFI1785w
PF3D7_0532200	PFE1595c, MAL5P1.313			27.3	226		Involved in response to chloroquine treatment (134, 135)
PF3D7_0731100	MAL7P1.172, GEXP11 PfPTP2	x		92.5	799	x	Involved in cell-cell communication and plasmid transfer, located on budding vesicles from Maurer's clefts (43); knockout with very reduced levels of surface PfEMP1, which was trapped in Maurer's clefts, no cytoadherence to CSA (44); indirect evidence for PfPTP2 being located inside the lumen of Maurer's clefts (44); coprecipitated with plasmepsin V (136); RESA-like protein found among soluble proteins from parasitophorous vacuole (PV) lumen and iRBC cytosol (82)
PF3D7_0801000	PF08_0137	x		147.1	1,127		Found among soluble proteins from PV lumen and iRBC cytosol (82), coprecipitated PTEX components such as HSP101 and PTEX150 (45), associated with J dots (J. Przyborski, personal communication), P. yoelii orthologue is annotated as a putative dentin phosphoryn protein possibly involved in mineral nucleation or functions as an extracellular matrix protein (137), contains a relaxed PEXEL motif (1), elicits an immune response (138)
PF3D7_0830600	MAL8P1.4	x		45.1	380		Located in Maurer's clefts, no interaction with the ATS of PfEMP1 (19); expression downregulated in HB3, 3D7, and Dd2 (28); expression upregulated with treatment with histone deacetylase inhibitors (trichostatin A, suberoylanilide hydroxamic acid) (139)
PF3D7_0936600	PFI1770w, GEXP5	x		25.2	212		Originally classified as PHISTb (15); reclassified as PHISTc (16); sole “PHISTb” that showed only cytosolic localization (20); upregulated in gametocytes (107, 140); earliest-known postinvasion gametocyte marker, expressed at 14 h postinvasion and independent of major gametocyte marker, cannot promote gametocyte maturation alone when other factors are not present (51)
PF3D7_0936800	PFI1780w	x		38.6	324		Protein of unknown function, several TM domains predicted (15); interacts with the ATS of PfEMP1 (56); located at the iRBC periphery with PfEMP1 but not cotransported with PfEMP1 (19); stuttering motif identified not found in any other PHIST protein (141); noncanonical PEXEL motif that is correctly cleaved (12); suggested presence in figure displaying exported proteins (100)
PF3D7_1001700	PF10_0021	x		27.6	226		Significantly downregulated after chloroquine treatment (80)
PF3D7_1001800	PF10_0022	x		23.8	204		Corrected gene model with adjusted exon and intron sizes (142, 143)
PF3D7_1016500	PF10_0161	x		81.3	675		Wrongly annotated as PF3D7_1016600 previously (15), evidence for sumoylation (144)
PF3D7_1016600	PF10_0161 or PF10_0161a	x		27.8	226		Had been annotated together with PF3D7_1016500 (15), evidence for sumoylation (144), IFA shows bright punctuate pattern in iRBCs (20)
PF3D7_1016700	PF10_0162			98.8	830
PF3D7_1016800	PF10_0163	x		30.0	241
PF3D7_1148700	PF11_0503, GEXP12	x		38.1	323
PF3D7_1200900	PFL0045c, MAL12P1.9	x		37.6	310		Noncanonical PEXEL, localizes to small dotted structures in iRBCs (12)

^aCurrent gene identification from PlasmoDB.

^bOther/previous names.

^cThe presence of a PEXEL motif is indicated by “x,” as identified in reference 15 or 1.

^dAnnotated as a pseudogene (PG) in PlasmoDB.

^eFor proteins with a PEXEL motif, molecular mass and length were calculated for the PEXEL-cleaved form of the protein (the molecular mass for PEXEL-cleaved proteins was calculated by using the ExPASy Web tool [http://web.expasy.org/compute_pi/]).

^fExisting viable knockout (KO) parasites or natural gene deletions are indicated by “x” (see reference 44 or the reference[s] indicated).

^gInformation or references referring to the corresponding gene or protein. LSAP2, liver stage-associated protein 2; SNPs, single-nucleotide polymorphisms.