TABLE 5.
PHISTa-like and PHIST proteins
| Gene identificationa | Other gene identification(s) or name(s)b | Presence of PEXELc | Gene statusd | Molecular mass (kDa)e | Length (aa)e | KOf | Description (reference[s] or source)g |
|---|---|---|---|---|---|---|---|
| PF3D7_0831300 | MAL8P1.205, GEXP13 | (x) | 93.0 | 770 | Classified as a PHIST protein (16), although no PHIST domain identified in InterPro; detected in early gametocytes (35); has a PEXEL motif (RKLSE), although this is not yet indicated in any publication | ||
| PF3D7_0831500 | 39.1 | 335 | |||||
| PF3D7_0831750 | PG | 34.0 | 288 | ||||
| PF3D7_0831900 | MAL7P1.230 | PG | 33.5 | 287 | x | Deletion in field samples from South America (116), no deletion in samples from Central America (117) | |
| PF3D7_0832200.1 | MAL7P1.225 | 33.5 | 286 | One of the earliest-upregulated genes in the asexual cycle (28), one splice form of MAL7P1.225 (GeneDB) | |||
| PF3D7_0832200.2 | MAL7P1.225 | 34.3 | 293 | Same as PF3D7_0832200.1 | |||
| PF3D7_0832300 | MAL7P1.224 | 30.8 | 259 | Upregulated during the commitment phase for the sexual developmental cycle (53, 145) | |||
| PF3D7_0832700 | MAL7P1.220 | PG | 30.8 | 260 | |||
| PF3D7_1000700 | PF10_0007, PF10_0008 | x | PG | 26.1 | 209 | Both old gene identifications have different domains (27), PHISTa protein with N-terminal PHIST domain (15) | |
| PF3D7_1201200 | PFL0060w, MAL12P1.12 | x | 16.0 | 134 | Downregulated in several strains (28), PHISTa-like protein (15) | ||
| PF3D7_1301300 | MAL13P1.58 | PG | 28.9 | 247 | Not located in telomeric region (27) | ||
| PF3D7_1372300 | 24.2 | 206 | |||||
| PF3D7_1477300 | PF14_0744, Pfg14-744 | x | 22.8 | 196 | Strongly upregulated at gametocytogenesis (27); expression peak in stage I gametocytes but also present at stage II (85, 145, 146); grouped as PHIST or PHISTa-like protein (1, 15, 16); tested as a gametocyte marker (129); expressed as early as in committed schizont stage, regulated by AP2-G, and HP1 is reported to have a silencing effect (54) | ||
| PF3D7_1477400 | PF14_0745 | 36.2 | 303 | Expressed in parasites committed to the sexual cycle (27), found in the nuclear proteomic fraction (147), classified as a PHIST protein (16) |
a
Current gene identification from PlasmoDB.
b
Other/previous names.
c
The presence of a PEXEL motif is indicated by “x,” as identified in reference 15 or 1. A newly identified PEXEL is indicated by “(x).”
d
Annotated as a pseudogene (PG) in PlasmoDB.
e
For proteins with a PEXEL motif, molecular mass and length were calculated for the PEXEL-cleaved form of the protein (the molecular mass for PEXEL-cleaved proteins was calculated by using the ExPASy Web tool [http://web.expasy.org/compute_pi/]).
f
Existing viable knockout (KO) parasites or natural gene deletions are indicated by “x” (see reference 44 or the reference[s] indicated).
g
Information or references referring to the corresponding gene or protein.