FIG 2.
Changes in rRNAs upon incubation of spores or purified RNA at high temperatures. (A) Purified spores of B. megaterium QMB1551 (wild type) or B. subtilis PS533 (wild type) prepared in liquid or on plates, respectively, were incubated in water for 20 h at various temperatures; spores were harvested and purified to remove any germinated spores or those that had lost Ca-DPA because of the heat treatments; and RNA was extracted, purified, and subjected to agarose gel electrophoresis as described in Materials and Methods. (B) Purified B. megaterium growing cell rRNA was incubated for 20 h in water (W) or buffer (B), and analyzed by agarose gel electrophoresis as described in Materials and Methods. The values to the left show the migration positions of RNA markers (lane M) in kilobases, and the arrows to the right show the migration positions of B. megaterium growing cell 23S and 16S rRNAs.