FIG 1.

M. xanthus transposon screen outline. A library of 6,000 M. xanthus transposon mutants was generated using the EZ-Tn5 transposable element. (I) To select for single M. xanthus colonies, cells were plated into Top agar on solid CYE agar plates containing kanamycin for selection and incubated for 5 to 6 days. (II) Individual colonies were transferred onto CYE kanamycin agar plates. (III) B. subtilis NCIB3610 prey cells were spotted onto CFL agar plates (predation/starvation medium), and the M. xanthus transposon mutants were transferred into the middle, showing the inside-out assay. Screening for predation phenotypes occurred for up to 5 days. GOF and LOF strains were selected by comparing them to M. xanthus wild type (WT) and an LOF control strain (mglAB mutant), as seen in the lower part of the figure.