FIG 5.

Knockout lethality predictions from running FBA on our models show close agreement with experimental results of hydrogenase knockouts. Green boxes indicate growth phenotypes where our models correctly replicated experimental results, red boxes indicate growth phenotypes where our models were incorrect, and white boxes indicate growth phenotypes where we lacked experimental validation data. Across the full spectrum of conditions, our models correctly predicted 28 of 30 conditions (93%), resulting in a strong Matthews correlation coefficient of 0.78. This suggests that our reconstruction produces models that accurately depict the effects of genotype alterations on growth phenotypes. L, lethal prediction; N, nonlethal prediction.