Figure 1.

A. Representative histology shown from individual and time-course experiments. From left to right, apoptotic and oncotic cells are observed in the cisplatinum and decay treatments, respectively. Note the disparity in cell organization and distribution of potential scatterers between the two mechanisms, with apoptosis displaying substantially greater arrangement of condensed chromatin at 72 hours. Heat death and colchicine treatments are shown on the right-most panels, where sudden death and mitotic arrest are displayed, respectively. B. Representative B-mode ultrasound scans at 20 MHz display the effects of morphological and structural changes to samples on the backscatter intensity. Notably, all cell forms of death involving condensation of DNA were associated with increases in B-mode speckle intensity (intensity scale bar). C. Cell and nuclei average diameter measurements for 72-hour cisplatinum, 36-hour colchicine, heat death, and 72-hour oncotic decay treatments. These data represent a mean of 40 measurements taken from 2 independent H&E stained slices per sample. Error bars represent SD. * indicates p<0.01 for whole cell measurements relative to the control. † indicates p<0.01 for nuclei measurements relative to the control nuclei.