Skip to main content
. 2016 Sep 12;3(9-10):275–287. doi: 10.18632/oncoscience.319

Figure 4. Flow cytometric differentiation of viable, apoptotic, and oncotic cells.

Figure 4

Following debris and doublet exclusion, cells were gated on the fluorescence viability indicator (propidium iodide), then backgated onto a colour dot plot, with viable cells represented as blue and non-viable represented as red. A. Untreated control samples generally contained viable cells which were characterized by relatively highly FSC and SSC. B. Cisplatinum treatment for 72 hours was followed by the appearance of an apoptotic-necrotic population featuring decreased FSC and slightly elevated SSC. C. Ischemic decay for 72 hours produced an oncotic-necrotic population featuring the vast majority of non-viable cells as having decreased both FSC and SSC. D. Scatterplot of average values for viable, apoptotic-necrotic, and oncotic-necrotic populations. All populations are visibility and statistically differentiable (p<0.01). Error bars represent SD for n=4 sample size.