Table 1.
Spectroscopic Properties of Fluorescent Bisphosphonate Probes
| probes | maximum absorption wavelengthb (λmax (abs), nm) |
maximum emission wavelengthb (λmax (em), nm) |
extinction coefficientc,d (M−1 cm−1) |
|---|---|---|---|
| 5(6)-FAM-RIS (7a1)a | 493 | 518 | 73,000 (pH 7.2) |
| 5-FAM-RIS (7a2)a | 493 | 521 | 73,000 (pH 7.2) |
| 6-FAM-RIS (7a3)a | 493 | 517 | 73,000 (pH 7.2) |
| 5(6)-RhR-RIS (7a4) | 567.5 | 589 | 114,850 (pH 7.5) |
| 5(6)-ROX-RIS (7a5) | 580 | 606 | 72,000 (pH 8.0) |
| AF647-RIS (7a6) | 648 | 666 | 240,000 (pH 7.0) |
| 5(6)-FAM-RISPC (5(6)-FAM-3-PEHPC, 7b1)a | 493 | 518 | 73,000 (pH 7.2) |
| 5(6)-RhR-RISPC (5(6)-RhR-3-PEHPC, 7b2) | 568 | 589 | 114,850 (pH 7.5) |
| 5(6)-ROX-RISPC (5(6)-ROX-3-PEHPC, 7b3) | 579 | 606 | 72,000 (pH 8.0) |
| AF647-RISPC (AF647–3-PEHPC, 7b4) | 648 | 666 | 240,000 (pH 7.0) |
| 5(6)-FAM-dRIS (7c1)a | 493 | 518 | 73,000 (pH 7.2) |
| 5(6)-RhR-dRIS (7c2) | 567.5 | 589 | 114,850 (pH 7.5) |
| 5-FAM-ZOL (7d1) | 493 | 521 | 73,000 (pH 7.2) |
| 6-FAM-ZOL (7d2) | 493 | 516 | 73,000 (pH 7.2) |
| AF647-ZOL (7d3) | 648.5 | 666 | 240,000 (pH 7.0) |
| 800CW-ZOL (7d4) | 774 | 789 | 240,000 (pH 7.0) |
| Sulfo-Cy5-ZOL (7d5) | 644 | 663 | 271,000 (1× PBS, pH 7.4) |
| 5-FAM-MIN (7e1) | 493 | 522 | 73,000 (pH 7.2) |
| 6-FAM-MIN (7e2) | 493 | 518 | 73,000 (pH 7.2) |
| 5-FAM-MINPC (5-FAM-3-IPEHPC, 7f1) | 493 | 522 | 73,000 (pH 7.2) |
| 6-FAM-MINPC (6-FAM-3-IPEHPC, 7f2) | 493 | 517 | 73,000 (pH 7.2) |
a
Previously reported.27
b
There is ±1 nm error in λmax (abs) and λmax (em).
c
The extinction coefficient of each probe is approximated as same as its corresponding fluorescent dye.
d
Unless specified, all measurements were carried out on samples in 0.1 M phosphate buffer.