Table 2.
Immune cell subpopulations and their surface markers.
| Immune cell subsets | Definition |
|---|---|
| Hematopoietic cells | CD45+ |
| B cells | CD45+, CD19+ |
| Myeloid dendritic cells | CD45+, CD11c+, CD19−, CD123− |
| Plasmacytoid dendritic cells | CD45+, CD11c−, CD19−, CD123+ |
| CD34+ progenitor cells | CD45+, CD34+ |
| CD3+ T cells | CD45+, CD3+ |
| CD8+ T cells | CD45+, CD3+, CD4−, CD8+ |
| CD4+ T cells | CD45+, CD3+, CD4+, CD8− |
| CD25+ T cells | CD45+, CD3+, CD4+, CD8−, CD25+ |
| CD3− cells | CD45+, CD3− |
| Natural killer cells | CD45+, CD3−, CD16+, CD56+ |
| Lymphocytes | FSC, SSC, CD45+, CD3+ |
| Monocytes | FSC, SSC, CD45+ |
Immune cell subpopulations were identified using combinations of fluorochrome-labeled antibodies. Cell populations were gated based on their combination of cell surface markers, cell size, and granularity.