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. 2016 Nov 1;143(21):3944–3955. doi: 10.1242/dev.138917

Fig. 3.

Fig. 3.

Rusc2 forms a heterotrimeric complex with Sufu and Gli proteins. (A) CoIP showing that FLAG-hRUSC2 interacts with myc-Gli1 (left), myc-Gli2 (middle) and myc-Gli3 (right). (B) CoIP showing lack of complex formation between overexpressed Rusc2 and Gli3 in Sufu knockout MEFs. (C) CoIP showing that hRUSC2 forms a complex with wild-type hSUFU, but not hSUFUR362C, which is deficient in binding Gli protein. (D) CoIP showing that overexpression of mouse Spop reduces the interaction between hSUFU and hRUSC2.